Journal: Journal of Proteome Research

Article Title: Accurate Peptide Fragment Mass Analysis: Multiplexed Peptide Identification and Quantification

doi: 10.1021/pr2008175

Figure Lengend Snippet: A) Experimental scan cycle for both DIA and DDA acquisitions B) Histogram of the identifications made using DDA mass spectrometry with Mascot versus DIA mass spectrometry with FT-ARM during equivalent acquisition periods. Inset shows identifications made when DIA acquisition window is reduced from 100 Da to 12 Da. Venn diagram inset showing the overlap between the identifications made with each approach at <5% estimated FDR. C) Histogram shows identifications made when DIA acquisition window is reduced from 100 Da to 12 Da. Venn diagram inset showing the overlap between the identifications made with each approach at <5% estimated FDR.

Article Snippet: Separate experiments were used for both DDA Masco (1292 unique peptides) and DIA FT-AR (896 unique peptides), with data acquired using the scan cycle shown in .

Techniques: Mass Spectrometry

Journal: Journal of Proteome Research

Article Title: Accurate Peptide Fragment Mass Analysis: Multiplexed Peptide Identification and Quantification

doi: 10.1021/pr2008175

Figure Lengend Snippet: A) Spectrum for which an FT-ARM unique identification was found. The overlapping isotope distributions shown did not yield identification with DDA methods. B) A plot showing the effect of the reduction of the DIA window size on the identifications per amu.

Article Snippet: Separate experiments were used for both DDA Masco (1292 unique peptides) and DIA FT-AR (896 unique peptides), with data acquired using the scan cycle shown in .

Techniques: